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To determine the difference between the two subjects exposed to the red and white light, I will perform chromatography. (Chromatography is a way to separate mixtures.) Then, with a spectrometer, I will examine the difference in color intensity, or concentration.
The purpose of this experiment was to find the difference in pigment concentration between two different types of plants, depending on two different sources of light. In which the clearlight and the red light take place. Inthis project, I required 2 red light bulbs and 1 clear light bulb per plant. I need two red bulbs because the ‘’red’’ energy transmitted is about half the amount the clear bulb produces. the box contains the plant. Specifically; I needed 2 of each plant, concluding, I will use overall, for the plant growth, 4 plants (2 of each), 4 boxes, and 12 bulbs (8 being red/4 being white). It will grow for one weks.
The Difference in Pigment Concentration When a Plant is Exposed to either a Red or Clear Light
Obtain all materials and bring them to your lab station. Cut chromatography paper in half lengthwise. Cut one end of the chromatography paper into a point or tip. Make a light pencil line about 1.0 cm from the end of the paper you just cut. Use the coin to extract plant pigments from the leaf. Take the leaf and place it over the pencil line you have drawn. Roll the penny over the leaf at the same position as the pencil line. You should see plant pigment across the pencil line you have drawn. Move the leaf around and repeat this step 8-10 times using different parts of the leaf. Pour about 1.0 cm of solvent into the bottom of the 600 mL beaker. Place the tip of the filter paper in the solvent being careful not to let the solvent touch the plant pigment line you have made. Place a watch glass over the 600 mL beaker. The solvent should travel up the filter paper. When the solvent is about 1-2 cm from the top of the filter paper remove the filter paper and make a pencil mark where the solvent stopped before the solvent evaporated. Allow the paper to dry. Once the paper dries, draw a pencil line at the bottom of each distinguishable pigment band on the filter paper. Depending on the leaf you used, you should see 4-5 bands. Repeat the procedure one more time with a different leaf or petal.
Materials: 600 mL beaker ,chromatography paper, chromatography solvent,ruler, pencil,penny, leaf (variety), scissors, watch glass, pH standards prepared in pre-activity, well plates, and pipet- Beral-type
Milanareaschools.org http://www.place.dawsoncollege.qc.ca/~drjes/issues/1/plants.html
